Mouse Monoclonal Antibody to CLEC2

Item Information

Catalog #

Size

Price

Description

Natural killer (NK) cells express multiple calcium-dependent (C-type) lectin-like receptors, such as CD94 (KLRD1; MIM 602894) and NKG2D (KLRC4; MIM 602893), that interact with major histocompatibility complex class I molecules and either inhibit or activate cytotoxicity and cytokine secretion. CLEC2 is a C-type lectin-like receptor expressed in myeloid cells and NK cells (Colonna et al., 2000 [PubMed 10671229])

Product Overview

Entrez GenelD

51266

Aliases

CLEC2; CLEC2B; PRO1384; QDED721; 1810061I13Rik

Clone#

4D2A6

Host / Isotype

Mouse / IgG2a

Immunogen

Purified recombinant fragment of human CLEC2 (AA: Extra(58-229)) expressed in E. Coli.

Formulation

Purified antibody in PBS with 0.05% sodium azide

Storage

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Product Applications

WB (Western Blot)

1/500 - 1/2000

IHC_P(Immunohistochemistry)

1/200 - 1/1000

FCM (Flow Cytometry)

1/200 - 1/400

ELISA

1/10000

References

1,Oncotarget. 2016 Sep 27;7(39):63661-63668. 2,Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2020 Oct;28(5):1643-1647.

Product Image

Elisa

Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Western Blot

Figure 2:Western blot analysis using CLEC2 mAb against human CLEC2 (AA: Extra(58-229)) recombinant protein. (Expected MW is 23.3 kDa)

Western Blot

Figure 3:Western blot analysis using CLEC2 mAb against HEK293-6e (1) and human CLEC2 (AA: Extra(58-229))-hIgGFc transfected HEK293-6e (2) cell lysate.

Flow cytometric analysis