Mouse Monoclonal Antibody to CLEC2
Item Information
Catalog #
Size
Price
Description
Natural killer (NK) cells express multiple calcium-dependent (C-type) lectin-like receptors, such as CD94 (KLRD1; MIM 602894) and NKG2D (KLRC4; MIM 602893), that interact with major histocompatibility complex class I molecules and either inhibit or activate cytotoxicity and cytokine secretion. CLEC2 is a C-type lectin-like receptor expressed in myeloid cells and NK cells (Colonna et al., 2000 [PubMed 10671229])
Product Overview
Entrez GenelD
51266
Aliases
CLEC2; CLEC2B; PRO1384; QDED721; 1810061I13Rik
Clone#
4D2A6
Host / Isotype
Mouse / IgG2a
Immunogen
Purified recombinant fragment of human CLEC2 (AA: Extra(58-229)) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4℃; -20℃ for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1,Oncotarget. 2016 Sep 27;7(39):63661-63668. 2,Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2020 Oct;28(5):1643-1647.
Product Image
Elisa
![Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/153649945f57fb6ba2a38c41e2849c6bfedc7352fa90ccf1cbc7523d6119dddc.jpg?v=1666705996)
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
![Figure 2:Western blot analysis using CLEC2 mAb against human CLEC2 (AA: Extra(58-229)) recombinant protein. (Expected MW is 23.3 kDa)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/20af5d781f2bba4d12a7022a583f3da8c1ce1502612f2f763a71f596a376af04.jpg?v=1666705996)
Figure 2:Western blot analysis using CLEC2 mAb against human CLEC2 (AA: Extra(58-229)) recombinant protein. (Expected MW is 23.3 kDa)
Western Blot
![Figure 3:Western blot analysis using CLEC2 mAb against HEK293-6e (1) and human CLEC2 (AA: Extra(58-229))-hIgGFc transfected HEK293-6e (2) cell lysate.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/e6fba7733834a2296b604c8a91c4418edef28c9526c41221197e4f0b9240b029.jpg?v=1666705996)
Figure 3:Western blot analysis using CLEC2 mAb against HEK293-6e (1) and human CLEC2 (AA: Extra(58-229))-hIgGFc transfected HEK293-6e (2) cell lysate.
Flow cytometric analysis
![Figure 4:Flow cytometric analysis of Jurkat cells using CLEC2 mouse mAb (green) and negative control (red).](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/ea4f1b4178bc2e34bd26f0b62db256e2c2e7f860d658139c58179dc1970e4021.jpg?v=1666705996)
Figure 4:Flow cytometric analysis of Jurkat cells using CLEC2 mouse mAb (green) and negative control (red).
Immunohistochemical analysis
![Figure 5:Immunohistochemical analysis of paraffin-embedded bladder Cancer tissues using CLEC2 mouse mAb with DAB staining.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/ec7f2f6316b01a57d62fb46b484e851f826c8ec0a85e3d6d795bec5ac05da4a4.jpg?v=1666705996)
Figure 5:Immunohistochemical analysis of paraffin-embedded bladder Cancer tissues using CLEC2 mouse mAb with DAB staining.
Immunohistochemical analysis
![Figure 6:Immunohistochemical analysis of paraffin-embedded liver cancer tissues using CLEC2 mouse mAb with DAB staining.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/1bbc6e2f08233a8394909404e33998f7a708b2ae2fc62a1d68910c4a2fc89448.jpg?v=1666705996)
Figure 6:Immunohistochemical analysis of paraffin-embedded liver cancer tissues using CLEC2 mouse mAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.