CK5 Primary Antibody
Item Information
Catalog #
Size
Price
Description
The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the basal layer of the epidermis with family member KRT14. Mutations in these genes have been associated with a complex of diseases termed epidermolysis bullosa simplex. The type II cytokeratins are clustered in a region of chromosome 12q12-q13.
Product Overview
Entrez GenelD
3852
Aliases
K5; KRT5; DDD; EBS2; KRT5A
Clone#
2C2B4
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human CK5 (AA: 316-590) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Am J Surg Pathol. 2009 Nov;33(11):1615-23.
2.J Dermatol. 2009 Aug;36(8):447-52.
2.J Dermatol. 2009 Aug;36(8):447-52.
Product Image
Western Blot
Figure 1: Western blot analysis using CK5 mAb against human CK5 recombinant protein. (Expected MW is 47.8 kDa)
Western Blot
Figure 2: Western blot analysis using CK5 mouse mAb against A431 (1), MCF-7 (2), Hela (3) and HepG2 (4) cell lysate.
Flow cytometric
Figure 3: Flow cytometric analysis of Hela cells using CK5 mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 4: Immunohistochemical analysis of paraffin-embedded endometrial cancer tissues using CK5 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 5: Immunohistochemical analysis of paraffin-embedded stomach tissues using CK5 mouse mAb with DAB staining.
Elisa
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
For Research Use Only. Not for use in diagnostic procedures.