CK5 Primary Antibody

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Description

The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the basal layer of the epidermis with family member KRT14. Mutations in these genes have been associated with a complex of diseases termed epidermolysis bullosa simplex. The type II cytokeratins are clustered in a region of chromosome 12q12-q13.

Product Overview
Entrez GenelD
3852
Aliases
KRT5; K5; DDD; EBS2; KRT5A
Clone#
2C2
Host / Isotype
Mouse / IgG1
Species Reactivity
Human, Mouse, Monkey
Immunogen
Purified recombinant fragment of human CK5 expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
ICC (Immunocytochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
Am J Surg Pathol. 2009 Nov;33(11):1615-23.
J Dermatol. 2009 Aug;36(8):447-52.
Product Image
Western Blot
Figure 1: Western blot analysis using CK5 mAb against human CK5 (AA: 158-272) recombinant protein. (Expected MW is 33.3 kDa)
Figure 1: Western blot analysis using CK5 mAb against human CK5 (AA: 158-272) recombinant protein. (Expected MW is 33.3 kDa)
Western Blot
Figure 2: Western blot analysis using CK5 mouse mAb against A431 (1), MCF-7 (2), HeLa (3), HepG2 (4), 3T3-L1 (5), and COS-7 (6) cell lysate.
Figure 2: Western blot analysis using CK5 mouse mAb against A431 (1), MCF-7 (2), HeLa (3), HepG2 (4), 3T3-L1 (5), and COS-7 (6) cell lysate.
Immunohistochemical analysis
Figure 3: Immunohistochemical analysis of paraffin-embedded breast cancer tissues using CK5 mouse mAb with DAB staining.
Figure 3: Immunohistochemical analysis of paraffin-embedded breast cancer tissues using CK5 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 4: Immunohistochemical analysis of paraffin-embedded stomach cancer tissues using CK5 mouse mAb with DAB staining.
Figure 4: Immunohistochemical analysis of paraffin-embedded stomach cancer tissues using CK5 mouse mAb with DAB staining.
Immunofluorescence analysis
Figure 5: Immunofluorescence analysis of HeLa cells. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Figure 5: Immunofluorescence analysis of HeLa cells. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Immunofluorescence analysis
Figure 6: Immunofluorescence analysis of HeLa cells using CK5 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Figure 6: Immunofluorescence analysis of HeLa cells using CK5 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Flow cytometric
Figure 7: Flow cytometric analysis of HeLa cells using CK5 mouse mAb (green) and negative control (red).
Figure 7: Flow cytometric analysis of HeLa cells using CK5 mouse mAb (green) and negative control (red).
Elisa
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
For Research Use Only. Not for use in diagnostic procedures.