CIRBP Primary Antibody
Item Information
Catalog #
Size
Price
Description
Cold-inducible mRNA binding protein that plays a protective role in the genotoxic stress response by stabilizing transcripts of genes involved in cell survival. Acts as a translational activator. Seems to play an essential role in cold-induced suppression of cell proliferation. Binds specifically to the 3-untranslated regions (3-UTRs) of stress-responsive transcripts RPA2 and TXN. Acts as a translational repressor (By similarity). Promotes assembly of stress granules (SGs), when overexpressed.
Product Overview
Entrez GenelD
1153
Aliases
CIRP
Clone#
6E12B3
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human CIRBP (AA: 1-90) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
ELISA
1/10000
References
1.Med Sci Monit. 2015 May 2;21:1256-60.
2.Int J Gynecol Pathol. 2003 Jul;22(3):240-7.
2.Int J Gynecol Pathol. 2003 Jul;22(3):240-7.
Product Image
Elisa
Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using CIRBP mAb against human CIRBP (AA: 1-90) recombinant protein. (Expected MW is 35.9 kDa)
Western Blot
Figure 3:Western blot analysis using CIRBP mAb against HEK293 (1) and CIRBP (AA: 1-90)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot
Figure 4:Western blot analysis using CIRBP mouse mAb against SW480 (1), PC-3 (2), A431 (3), and U251 (4) cell lysate.
Immunohistochemical analysis
Figure 5:Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using CIRBP mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 6:Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using CIRBP mouse mAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.