CIDEC Primary Antibody

Item Information

Catalog #

Size

Price

Description

This gene encodes a member of the cell death-inducing DNA fragmentation factor-like effector family. Members of this family play important roles in apoptosis. The encoded protein promotes lipid droplet formation in adipocytes and may mediate adipocyte apoptosis. This gene is regulated by insulin and its expression is positively correlated with insulin sensitivity. Mutations in this gene may contribute to insulin resistant diabetes. A pseudogene of this gene is located on the short arm of chromosome 3. Alternatively spliced transcript variants that encode different isoforms have been observed for this gene.

Product Overview

Entrez GenelD

63924

Aliases

CIDE3; FPLD5; FSP27; CIDE-3

Clone#

7C12F11

Host / Isotype

Mouse / IgG1

Species Reactivity

Human

Immunogen

Purified recombinant fragment of human CIDEC (AA: 53-141) expressed in E. Coli.

Formulation

Purified antibody in PBS with 0.05% sodium azide

Storage

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Product Applications

WB (Western Blot)

1/500 - 1/2000

ICC (Immunocytochemistry)

1/200 - 1/1000

FCM (Flow Cytometry)

1/200 - 1/400

ELISA

1/10000

References

1.Obesity (Silver Spring). 2010 Feb;18(2):417-9.
2.EMBO Mol Med. 2009 Aug;1(5):280-7.

Product Image

Elisa

Figure 1: Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);

Western Blot

Figure 2:Western blot analysis using CIDEC mAb against human CIDEC (AA: 53-141) recombinant protein. (Expected MW is 36 kDa)

Western Blot

Figure 3:Western blot analysis using CIDEC mAb against HEK293 (1) and CIDEC (AA: 53-141)-hIgGFc transfected HEK293 (2) cell lysate.

Western Blot

Figure 4:Western blot analysis using CIDEC mouse mAb against HEK293 (1), A431 (2), and HCT116 (3) cell lysate.

Immunofluorescence analysis

Figure 5:Immunofluorescence analysis of Hela cells using CIDEC mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Flow cytometric