CHUK Primary Antibody
Item Information
Catalog #
Size
Price
Description
This gene encodes a member of the serine/threonine protein kinase family. The encoded protein, a component of a cytokine-activated protein complex that is an inhibitor of the essential transcription factor NF-kappa-B complex, phosphorylates sites that trigger the degradation of the inhibitor via the ubiquination pathway, thereby activating the transcription factor.
Product Overview
Entrez GenelD
1147
Aliases
IKK1; IKKA; IKBKA; TCF16; NFKBIKA; IKK-alpha; CHUK
Clone#
3G12H9
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human CHUK expressed in E. Coli.
Formulation
Ascitic fluid containing 0.03% sodium azide.
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Product Applications
WB (Western Blot)
1/500 - 1/2000
ICC (Immunocytochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1. Mol Cancer. 2010 Jan 5;9:1.
2. J Infect Dis. 2010 May 1;201(9):1371-80.
2. J Infect Dis. 2010 May 1;201(9):1371-80.
Product Image
Western Blot
Figure 1: Western blot analysis using CHUK mouse mAb against Raji (1), Jurkat (2), THP-1 (3) and K562 (4) cell lysate.
Immunofluorescence analysis
Figure 2: Immunofluorescence analysis of NIH/3T3 cells using CHUK mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Flow cytometric
Figure 3: Flow cytometric analysis of A549 cells using CHUK mouse mAb (blue) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.