CHRND Primary Antibody
Item Information
Catalog #
Size
Price
Description
The acetylcholine receptor of muscle has 5 subunits of 4 different types: 2 alpha and 1 each of beta, gamma and delta subunits. After acetylcholine binding, the receptor undergoes an extensive conformation change that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. Defects in this gene are a cause of multiple pterygium syndrome lethal type (MUPSL), congenital myasthenic syndrome slow-channel type (SCCMS), and congenital myasthenic syndrome fast-channel type (FCCMS). Several transcript variants encoding different isoforms have been found for this gene.
Product Overview
Entrez GenelD
1144
Aliases
ACHRD; CMS2A; CMS3A; CMS3B; CMS3C; FCCMS; SCCMS
Clone#
1H1F9
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human CHRND (AA: extra 22-245) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Product Applications
WB (Western Blot)
1/500 - 1/2000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Clin Dysmorphol. 2013 Apr;22(2):54-8.
2.J Biol Chem. 2014 Jan 3;289(1):203-14.
2.J Biol Chem. 2014 Jan 3;289(1):203-14.
Product Image
Elisa
Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using CHRND mAb against human CHRND (AA: extra 22-245) recombinant protein. (Expected MW is 52.2 kDa)
Western Blot
Figure 3:Western blot analysis using CHRND mAb against HEK293 (1) and CHRND (AA: extra 22-245)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot
Figure 4:Western blot analysis using CHRND mouse mAb against C6 (1) cell lysate.
Flow cytometric
Figure 5:Flow cytometric analysis of SK-N-SH cells using CHRND mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.