CHRNA7 Primary Antibody
The nicotinic acetylcholine receptors (nAChRs) are members of a superfamily of ligand-gated ion channels that mediate fast signal transmission at synapses. The nAChRs are thought to be hetero-pentamers composed of homologous subunits. The proposed structure for each subunit is a conserved N-terminal extracellular domain followed by three conserved transmembrane domains, a variable cytoplasmic loop, a fourth conserved transmembrane domain, and a short C-terminal extracellular region. The protein encoded by this gene forms a homo-oligomeric channel, displays marked permeability to calcium ions and is a major component of brain nicotinic receptors that are blocked by, and highly sensitive to, alpha-bungarotoxin. Once this receptor binds acetylcholine, it undergoes an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. This gene is located in a region identified as a major susceptibility locus for juvenile myoclonic epilepsy and a chromosomal location involved in the genetic transmission of schizophrenia. An evolutionarily recent partial duplication event in this region results in a hybrid containing sequence from this gene and a novel FAM7A gene. Alternative splicing results in multiple transcript variants.
Purified antibody in PBS with 0.05% sodium azide
4°C; -20°C for long term storage
Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 2:Western blot analysis using CHRNA7 mAb against human CHRNA7 (AA: extra 52-259) recombinant protein. (Expected MW is 50.4 kDa)
Figure 3:Western blot analysis using CHRNA7 mAb against HEK293 (1) and CHRNA7 (AA: extra 52-259)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 4:Western blot analysis using CHRNA7 mouse mAb against membrane protein lysate of C6 (1), membrane protein lysate of SK-N-SH (2), C6 (3), and HepG2 (4) cell lysate.
Figure 5:Flow cytometric analysis of SH-SY5Y cells using CHRNA7 mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.