CHRNA5 Primary Antibody

Item Information

Catalog #

Size

Price

Description

The protein encoded by this gene is a nicotinic acetylcholine receptor subunit and a member of a superfamily of ligand-gated ion channels that mediate fast signal transmission at synapses. These receptors are thought to be heteropentamers composed of separate but similar subunits. Defects in this gene have been linked to susceptibility to lung cancer type 2 (LNCR2).

Product Overview

Entrez GenelD

1138

Aliases

LNCR2

Clone#

2D2D10

Host / Isotype

Mouse / IgG1

Species Reactivity

Human, Rat

Immunogen

Purified recombinant fragment of human CHRNA5 (AA: extra 23-254) expressed in E. Coli.

Formulation

Purified antibody in PBS with 0.05% sodium azide

Storage

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Product Applications

WB (Western Blot)

1/500 - 1/2000

FCM (Flow Cytometry)

1/200 - 1/400

ELISA

1/10000

References

1.Asian Pac J Cancer Prev. 2015;16(15):6685-90.
2.Nicotine Tob Res. 2016 Feb;18(2):212-20.

Product Image

Elisa

Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Western Blot

Figure 2:Western blot analysis using CHRNA5 mAb against human CHRNA5 (AA: 23-254) recombinant protein. (Expected MW is 52.5 kDa)

Western Blot

Figure 3:Western blot analysis using CHRNA5 mAb against HEK293 (1) and CHRNA5 (AA: 23-254)-hIgGFc transfected HEK293 (2) cell lysate.

Western Blot

Figure 4:Western blot analysis using CHRNA5 mouse mAb against membrane protein lysate of C6 (1), membrane protein lysate of SK-N-SH (2), membrane protein lysate of C6 (3), and C6 (4) cell lysate.

Flow cytometric