CHRM5 Primary Antibody
Item Information
Catalog #
Size
Price
Description
The protein encoded by this gene is structurally similar to G protein-coupled receptors and is highly expressed in endothelial cells. It binds the ligand sphingosine-1-phosphate with high affinity and high specificity, and suggested to be involved in the processes that regulate the differentiation of endothelial cells. Activation of this receptor induces cell-cell adhesion. Alternative splicing results in multiple transcript variants.
Product Overview
Entrez GenelD
1133
Aliases
HM5
Clone#
7E5H11
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human CHRM5 expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Product Applications
WB (Western Blot)
1/500 - 1/2000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.J R Soc Interface. 2016 Dec;13(125). pii: 20160823.
2.Eur J Cancer. 2015 Sep;51(14):1937-45.
2.Eur J Cancer. 2015 Sep;51(14):1937-45.
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using CHRM5 mAb against human CHRM5 recombinant protein. (Expected MW is 37.4 kDa)
Western Blot
Figure 3:Western blot analysis using CHRM5 mAb against HEK293 (1) and CHRM5-hIgGFc transfected HEK293 (2) cell lysate.
Flow cytometric
Figure 4:Flow cytometric analysis of HL-60 cells using CHRM5 mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.