CHK1 Primary Antibody

Item Information

Catalog #

Size

Price

Description

CHK1, also known as CHEK1, it is a kinase that phosphorylates cdc25, an important phosphatase in cell cycle control, particularly for entry into mitosis. Cdc25, when phosphorylated on serine 216 by chk1 becomes bound by an adaptor protein in the cytoplasm. Therefore it is inhibited from removing the inhibiting phosphate from MPF (mitotic/maturation promoting factor) added by Wee1. Consequently, a cell is prevented from entering mitosis. Chk1 can also phosphorylate p53 at Ser20 in vitro.

Product Overview

Entrez GenelD

1111

Aliases

CHEK1

Clone#

2G1D5

Host / Isotype

Mouse / IgG1

Species Reactivity

Human, Mouse

Immunogen

Purified recombinant fragment of CHK1 expressed in E. Coli.

Formulation

Ascitic fluid containing 0.03% sodium azide.

Storage

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Product Applications

WB (Western Blot)

1/500 - 1/2000

ELISA

1/10000

References

1. EMBO J. 1998. 16: 545-554.
2. Science 1998. 282: 1893-1897.

Product Image

Western Blot

Figure 1: Western blot analysis using CHK1 mouse mAb against A431 (1), Hela (2), NIH/3T3 (3) and K562 (4) cell lysate.

Western Blot

Figure 2: Western blot analysis using CHK1 mouse mAb against HEK293T cells transfected with the pCMV6-ENTRY control (1) and pCMV6-ENTRY CHK1 cDNA (2).

For Research Use Only. Not for use in diagnostic procedures.