CHGA Primary Antibody
Item Information
Catalog #
Size
Price
Description
The protein encoded by this gene is a member of the chromogranin/secretogranin family of neuroendocrine secretory proteins. It is found in secretory vesicles of neurons and endocrine cells. This gene product is a precursor to three biologically active peptides; vasostatin, pancreastatin, and parastatin. These peptides act as autocrine or paracrine negative modulators of the neuroendocrine system. Other peptides, including chromostatin, beta-granin, WE-14 and GE-25, are also derived from the full-length protein. However, biological activities for these molecules have not been shown.
Product Overview
Entrez GenelD
1113
Aliases
CGA
Clone#
1A6B6
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human CHGA (AA: 87-252 ) expressed in E. Coli.
Formulation
Ascitic fluid containing 0.03% sodium azide.
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
ICC (Immunocytochemistry)
1/50
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Cell Signal. 2012 Jan;24(1):233-46.
2.J Endocrinol Invest. 2010 Mar;33(3):186-91.
2.J Endocrinol Invest. 2010 Mar;33(3):186-91.
Product Image
Western Blot
Figure 1: Western blot analysis using CHGA mAb against human CHGA recombinant protein. (Expected MW is 43.6 kDa)
Western Blot
Figure 2: Western blot analysis using CHGA mouse mAb against MOLT4 (1), SK-N-SH (2), HepG2 (3), and PC-12 (4) cell lysate.
Immunofluorescence analysis
Figure 3: Immunofluorescence analysis of HepG2 cells using CHGA mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Flow cytometric
Figure 4: Flow cytometric analysis of HeLa cells using CHGA mouse mAb (green) and negative control (purple).
Elisa
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
For Research Use Only. Not for use in diagnostic procedures.