CEACAM5 Primary Antibody
Item Information
Catalog #
Size
Price
Description
This gene encodes a cell surface glycoprotein that represents the founding member of the carcinoembryonic antigen (CEA) family of proteins. The encoded protein is used as a clinical biomarker for gastrointestinal cancers and may promote tumor development through its role as a cell adhesion molecule. Additionally, the encoded protein may regulate differentiation, apoptosis, and cell polarity. This gene is present in a CEA family gene cluster on chromosome 19. Alternative splicing results in multiple transcript variants.
Product Overview
Entrez GenelD
1048
Aliases
CEA; CD66e
Clone#
3B9C9
Host / Isotype
Mouse / IgG2a
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human CEACAM5 (AA: 35-165) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Product Applications
WB (Western Blot)
1/500 - 1/2000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.J Virol. 2016 Sep 29;90(20):9114-27.
2.Asian Pac J Cancer Prev. 2016;17(S3):77-80.
2.Asian Pac J Cancer Prev. 2016;17(S3):77-80.
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using CEACAM5 mAb against human CEACAM5 (AA: 35-165) recombinant protein. (Expected MW is 40.5 kDa)
Western Blot
Figure 3:Western blot analysis using CEACAM5 mAb against HEK293 (1) and CEACAM5 (AA: 35-165)-hIgGFc transfected HEK293 (2) cell lysate.
Flow cytometric
Figure 4:Flow cytometric analysis of K562 cells using CEACAM5 mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.