Mouse Monoclonal Antibody to CEACAM3
Item Information
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Price
Description
This gene encodes a member of the family of carcinoembryonic antigen-related cell adhesion molecules (CEACAMs), which are used by several bacterial pathogens to bind and invade host cells. The encoded transmembrane protein directs phagocytosis of several bacterial species that is dependent on the small GTPase Rac. It is thought to serve an important role in controlling human-specific pathogens by the innate immune system. Alternatively spliced transcript variants have been described.
Product Overview
Entrez GenelD
1084
Aliases
CEA; CGM1; W264; W282; CD66D
Clone#
8D5F12
Host / Isotype
Mouse / IgG1
Immunogen
Purified recombinant fragment of human CEACAM3 (AA: 35-155) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4℃; -20℃ for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.J Huazhong Univ Sci Technolog Med Sci. 2016 Jun;36(3):344-349. 2.Mediators Inflamm. 2015;2015:931784.
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using CEACAM3 mAb against human CEACAM3 (AA: 35-155) recombinant protein. (Expected MW is 39 kDa)
Western Blot
Figure 3:Western blot analysis using CEACAM3 mAb against HEK293-6e (1) and CEACAM3 (AA: 35-155)-hIgGFc transfected HEK293-6e (2) cell lysate.
Western Blot
Figure 4:Western blot analysis using CEACAM3 mouse mAb against rat spleen (1) and rat lung (2) tissue lysate.
Flow cytometric analysis
Figure 5:Flow cytometric analysis of THP-1 cells using CEACAM3 mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.