CDKN1C Primary Antibody
Item Information
Catalog #
Size
Price
Description
This gene is imprinted, with preferential expression of the maternal allele. The encoded protein is a tight-binding, strong inhibitor of several G1 cyclin/Cdk complexes and a negative regulator of cell proliferation. Mutations in this gene are implicated in sporadic cancers and Beckwith-Wiedemann syndorome, suggesting that this gene is a tumor suppressor candidate. Three transcript variants encoding two different isoforms have been found for this gene.
Product Overview
Entrez GenelD
1028
Aliases
BWS; WBS; p57; BWCR; KIP2; p57Kip2
Clone#
8G5D5
Host / Isotype
Mouse / Mouse IgG1
Immunogen
Purified recombinant fragment of human CDKN1C expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200-1/1000
FCM (Flow Cytometry)
1/200-1/400
ELISA
1/10000
References
1.Liver Int. 2019 Jan;39(1):147-157. 2.Int J Mol Sci. 2018 Apr 2;19(4):1055.
Product Image
ELISA
Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng)
WESTERN BLOT
Figure 2: Western blot analysis using CDKN1C mAb against human CDKN1C recombinant protein. (Expected MW is 23.3 kDa)
WESTERN BLOT
Figure 3: Western blot analysis using CDKN1C mAb against HEK293-6e (1) and CDKN1C-hIgGFc transfected HEK293-6e (2) cell lysate.
WESTERN BLOT
Figure 4: Western blot analysis using CDKN1C mouse mAb against SK-Br-3 (1) cell lysate.
FLOW CYTOMETRY
Figure 5: Flow cytometric analysis of Hela cells using CDKN1C mouse mAb (green) and negative control (red).
IMMUNOHISTOCHEMISTRY
Figure 6: Immunohistochemical analysis of paraffin-embedded testis tissues using CDKN1C mouse mAb with DAB staining.
FLOW CYTOMETRY
Figure 7: Flow cytometric analysis of MCF-7 cells using CDKN1C mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.