Mouse Monoclonal Antibody to CDKN1A
This gene encodes a potent cyclin-dependent kinase inhibitor. The encoded protein binds to and inhibits the activity of cyclin-cyclin-dependent kinase2 or -cyclin-dependent kinase4 complexes, and thus functions as a regulator of cell cycle progression at G1. The expression of this gene is tightly controlled by the tumor suppressor protein p53, through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. This protein can interact with proliferating cell nuclear antigen, a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair. This protein was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of cyclin-dependent kinase2, and may be instrumental in the execution of apoptosis following caspase activation. Mice that lack this gene have the ability to regenerate damaged or missing tissue. Multiple alternatively spliced variants have been found for this gene.
![Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/cc1827b6caaa72cda84fb794d2be685a5c29e6f85d132e9ac1bd72ad26274a96.jpg?v=1666705279)
![Figure 2:Western blot analysis using CDKN1A mAb against human CDKN1A (AA: 1-164) recombinant protein. (Expected MW is 43.7 kDa)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/f73b54183b6faa993f058fb7a8617daf90deeb7dde239c58521ed2936466256d.jpg?v=1666705279)
![Figure 3:Western blot analysis using CDKN1A mAb against HEK293-6e (1) and CDKN1A (AA: 1-164)-hIgGFc transfected HEK293-6e (2) cell lysate.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/b65929f344d0b6b9eba8645b76b383aec5caa4506746012d70c1ab3d54c70f30.jpg?v=1666705279)
![Figure 4:Flow cytometric analysis of K562 cells using CDKN1A mouse mAb (green) and negative control (red).](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/950eebd797aa578e6174eccd2e8cf9a1cf16d6eb5886e79ebf6c0ecfe4f68580.jpg?v=1666705279)
![Figure 5:Immunohistochemical analysis of paraffin-embedded lung cancer tissues using CDKN1A mouse mAb with DAB staining.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/841bc4dcf5bfd80e879bdf4731336f30ef3835491bbc7a7fb903b398a8ba314c.jpg?v=1666705279)
![Figure 6:Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using CDKN1A mouse mAb with DAB staining.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/f5ebdae8944323197a4919b62ee73340fcdc9f4de53675eceb24e2190f1057e6.jpg?v=1666705279)