Mouse Monoclonal Antibody to CDK4

Item Information

Catalog #

Size

Price

Description

The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is highly similar to the gene products of S. cerevisiae cdc28 and S. pombe cdc2. It is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression. The activity of this kinase is restricted to the G1-S phase, which is controlled by the regulatory subunits D-type cyclins and CDK inhibitor p16(INK4a). This kinase was shown to be responsible for the phosphorylation of retinoblastoma gene product (Rb). Mutations in this gene as well as in its related proteins including D-type cyclins, p16(INK4a) and Rb were all found to be associated with tumorigenesis of a variety of cancers. Multiple polyadenylation sites of this gene have been reported.

Product Overview

Entrez GenelD

1019

Aliases

CMM3; PSK-J3

Clone#

4A11E2

Host / Isotype

Mouse / IgG1

Immunogen

Purified recombinant fragment of human CDK4 (AA: 77-303) expressed in E. Coli.

Formulation

Purified antibody in PBS with 0.05% sodium azide

Storage

4℃; -20℃ for long term storage

Product Applications

WB (Western Blot)

1/500 - 1/2000

IHC_P(Immunohistochemistry)

1/200 - 1/1000

ICC (Immunocytochemistry)

1/200 - 1/1000

FCM (Flow Cytometry)

1/200 - 1/400

ELISA

1/10000

References

1,Cancer Res. 2019 Oct 15;79(20):5245-5259. 2,J Dermatol. 2019 May;46(5):444-448

Product Image

Elisa

Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Western Blot

Figure 2:Western blot analysis using CDK4 mAb against human CDK4 (AA: 77-303) recombinant protein. (Expected MW is 51.6 kDa)

Western Blot

Figure 3:Western blot analysis using CDK4 mAb against HEK293-6e (1) and CDK4 (AA: 77-303)-hIgGFc transfected HEK293-6e (2) cell lysate.

Flow cytometric analysis

Figure 4:Flow cytometric analysis of K562 cells using CDK4 mouse mAb (green) and negative control (red).

Flow cytometric analysis

Figure 5:Flow cytometric analysis of U937 cells using CDK4 mouse mAb (green) and negative control (red).

Immunohistochemical analysis

Figure 6:Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using CDK4 mouse mAb with DAB staining.

Immunohistochemical analysis

Figure 7:Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using CDK4 mouse mAb with DAB staining.

Immunofluorescence analysis

Figure 8:Immunofluorescence analysis of Hela cells using CDK4 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

For Research Use Only. Not for use in diagnostic procedures.