CDK4 Primary Antibody
Item Information
Catalog #
Size
Price
Description
The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is highly similar to the gene products of S. cerevisiae cdc28 and S. pombe cdc2. It is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression. The activity of this kinase is restricted to the G1-S phase, which is controlled by the regulatory subunits D-type cyclins and CDK inhibitor p16(INK4a). This kinase was shown to be responsible for the phosphorylation of retinoblastoma gene product (Rb). Mutations in this gene as well as in its related proteins including D-type cyclins, p16(INK4a) and Rb were all found to be associated with tumorigenesis of a variety of cancers. Multiple polyadenylation sites of this gene have been reported.
Product Overview
Entrez GenelD
1019
Aliases
CMM3; PSK-J3
Clone#
4C5H9
Host / Isotype
Mouse / IgG1
Species Reactivity
Human, Mouse
Immunogen
Purified recombinant fragment of human CDK4 expressed in E. Coli.
Formulation
Ascitic fluid containing 0.03% sodium azide.
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.FEBS Lett. 2012 Feb 17;586(4):442-7.
2.J Transl Med. 2011 Apr 11;9:38.
2.J Transl Med. 2011 Apr 11;9:38.
Product Image
Western Blot
Figure 1: Western blot analysis using CDK4 mouse mAb against HeLa (1) and F9 (2) cell lysate.
Flow cytometric
Figure 2: Flow cytometric analysis of Hela cells using CDK4 mouse mAb (green) and negative control (purple).
Immunohistochemical analysis
Figure 3: Immunohistochemical analysis of paraffin-embedded testis tissues using CDK4 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 4: Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using CDK4 mouse mAb with DAB staining.
Elisa
Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);
For Research Use Only. Not for use in diagnostic procedures.