CDH5 Primary Antibody
Item Information
Catalog #
Size
Price
Description
This gene is a classical cadherin from the cadherin superfamily and is located in a six-cadherin cluster in a region on the long arm of chromosome 16 that is involved in loss of heterozygosity events in breast and prostate cancer. The encoded protein is a calcium-dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Functioning as a classic cadherin by imparting to cells the ability to adhere in a homophilic manner, the protein may play an important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. An alternative splice variant has been described but its full length sequence has not been determined.
Product Overview
Entrez GenelD
1003
Aliases
7B4; CD144
Clone#
3D5C7
Host / Isotype
Mouse / IgG1
Species Reactivity
Human, Rat
Immunogen
Purified recombinant fragment of human CDH5 (AA: 29-223 ) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Product Applications
WB (Western Blot)
1/500 - 1/2000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Blood. 2011 Feb 24;117(8):2515-26.
2.J Virol. 2010 Nov;84(21):11227-34.
2.J Virol. 2010 Nov;84(21):11227-34.
Product Image
Western Blot
Figure 1: Western blot analysis using CDH5 mAb against human CDH5 recombinant protein. (Expected MW is 47.6 kDa)
Western Blot
Figure 2: Western blot analysis using CDH5 mouse mAb against MCF-7 (1), A549 (2), HUVE-12 (3) cell lysate, and rat lung (4) tissue lysate.
Flow cytometric
Figure 3: Flow cytometric analysis of Jurkat cells using CDH5 mouse mAb (green) and negative control (purple).
Elisa
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
For Research Use Only. Not for use in diagnostic procedures.