CDH2 Primary Antibody
Item Information
Catalog #
Size
Price
Description
This gene is a classical cadherin from the cadherin superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. The protein functions during gastrulation and is required for establishment of left-right asymmetry. At certain central nervous system synapses, presynaptic to postsynaptic adhesion is mediated at least in part by this gene product.
Product Overview
Entrez GenelD
1000
Aliases
CDHN; NCAD; CD325; CDw325; CDH2
Clone#
5D5
Host / Isotype
Mouse / IgG1
Species Reactivity
Human, Mouse, Rat
Immunogen
Purified recombinant fragment of human CDH2 expressed in E. Coli.
Formulation
Ascitic fluid containing 0.03% sodium azide.
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
ICC (Immunocytochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1. J Biol Chem. 2007 Mar 16;282(11):8545-56.
2. Mol Cell Biochem. 2007 Aug;302(1-2):19-26.
3. Urol Oncol. 2010 Mar-Apr;28(2):180-8.
2. Mol Cell Biochem. 2007 Aug;302(1-2):19-26.
3. Urol Oncol. 2010 Mar-Apr;28(2):180-8.
Product Image
Western Blot
Figure 1: Western blot analysis using CDH2 mouse mAb against A431 (1), NIH/3T3 (2), Hela (3), C6 (4) and LNCap (5) cell lysate.
Immunohistochemical analysis
Figure 2: Immunohistochemical analysis of paraffin-embedded human lung cancer (A), colon cancer (B), ovarian cancer (C) and mammary cancer(D), using CDH2 mouse mAb with DAB staining.
Immunofluorescence analysis
Figure 3: Immunofluorescence analysis of A431 cells using CDH2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.
Immunohistochemical analysis
Figure 3: Immunohistochemical analysis of paraffin-embedded human Placenta tissues using CDH2 mouse mAb
Flow cytometric
Figure 4: Flow cytometric analysis of PC-2 cells using CDH2 mouse mAb (green) and negative control (purple).
For Research Use Only. Not for use in diagnostic procedures.