CDH16 Primary Antibody

Item Information
Catalog #Size/ConcentrationPrice
Specification
AliasesKSP-Cadherin
ProductOrderC
Clone#6H6E2
Entrez GenelD1014
FormulationPurified antibody in PBS with 0.05% sodium azide.
HostMouse
IsotypeIgG1
ImmunogenPurified recombinant fragment of human CDH16 (AA: EX(558-721)) expressed in E. Coli.
MW90kDa
Shipping InformationThis product will ship in a box containing blue ice at a temperature of 4°C. Learn More
Species ReactivityHuman
Application
ELISA1/10000
ICC (Immunocytochemistry)1/200 - 1/1000
IHC_P(Immunohistochemistry)1/200 - 1/1000
FCM (Flow Cytometry)1/200 - 1/400
WB (Western Blot)1/500 - 1/2000
Sequence
EX(558-721)
Catalog#: 30659
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Images
Western Blot
Figure 1: Western blot analysis using CDH16 mAb against human CDH16 (AA: EX(558-721)) recombinant protein. (Expected MW is 43.6 kDa)
Figure 1: Western blot analysis using CDH16 mAb against human CDH16 (AA: EX(558-721)) recombinant protein. (Expected MW is 43.6 kDa)
Western Blot
Figure 2: Western blot analysis using CDH16 mAb against HEK293 (1) and CDH16 (AA: EX(558-721))-hIgGFc transfected HEK293 (2) cell lysate.
Figure 2: Western blot analysis using CDH16 mAb against HEK293 (1) and CDH16 (AA: EX(558-721))-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot
Figure 3: Western blot analysis using CDH16 mouse mAb against A431 (1), Jurkat (2) cell lysate.
Figure 3: Western blot analysis using CDH16 mouse mAb against A431 (1), Jurkat (2) cell lysate.
Immunofluorescence analysis
Figure 4: Immunofluorescence analysis of A431 cells using CDH16 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Figure 4: Immunofluorescence analysis of A431 cells using CDH16 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric
Figure 5: Flow cytometric analysis of A431 cells using CDH16 mouse mAb (green) and negative control (red).
Figure 5: Flow cytometric analysis of A431 cells using CDH16 mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 6: Immunohistochemical analysis of paraffin-embedded kidney tissues using CDH16 mouse mAb with DAB staining.
Figure 6: Immunohistochemical analysis of paraffin-embedded kidney tissues using CDH16 mouse mAb with DAB staining.
Elisa
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Product Overview
Description

This gene is a member of the cadherin superfamily, genes encoding calcium-dependent, membrane-associated glycoproteins. Mapped to a previously identified cluster of cadherin genes on chromosome 16q22.1, the gene localizes with superfamily members CDH1, CDH3, CDH5, CDH8 and CDH11. The protein consists of an extracellular domain containing 6 cadherin domains, a transmembrane region and a truncated cytoplasmic domain but lacks the prosequence and tripeptide HAV adhesion recognition sequence typical of most classical cadherins. Expression is exclusively in kidney, where the protein functions as the principal mediator of homotypic cellular recognition, playing a role in the morphogenic direction of tissue development. Alternatively spliced transcript variants encoding distinct isoforms have been identified.

References (references)
References (references)1. Br J Cancer. 2005 Jun 6;92(11):2010-7.
2. Am J Physiol. 1999 Jul;277(1 Pt 2):F146-56.