CDC27 Primary Antibody
Item Information
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Price
Description
Cdc27 shares strong similarity with Saccharomyces cerevisiae protein Cdc27, and the gene product of Schizosaccharomyces pombe nuc 2. It is a component of the Anaphase Promoting Complex (APC), which is composed of eight protein subunits and is highly conserved in eucaryotic cells. The APC catalyzes the formation of the cyclin B ubiquitin conjugate that is responsible for the ubiquitin mediated proteolysis of B type cyclins. This protein and 3 other members of the APC complex contain the TPR (tetratricopeptide repeat), a protein domain important for protein protein interaction. This protein was shown to interact with mitotic checkpoint proteins including Mad2, p55CDC and BUBR1, and thus may be involved in controlling the timing of mitosis.
Product Overview
Entrez GenelD
996
Aliases
APC3; HNUC; ANAPC3; CDC27Hs; D0S1430E; D17S978E;
Clone#
5C12
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human CDC27 expressed in E. Coli.
Formulation
Ascitic fluid containing 0.03% sodium azide.
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
ELISA
1/10000
References
1. Mol Cell Biol. 2004 May;24(9):3577-87.
2. J Biol Chem. 2004 Oct 1;279(40):42128-38.
2. J Biol Chem. 2004 Oct 1;279(40):42128-38.
Product Image
Western Blot
Figure 1: Western blot analysis using CDC27 mouse mAb against CDC27(AA: 724-830)-hIgGFc transfected HEK293 cell.
Immunohistochemical analysis
Figure 2: Immunohistochemical analysis of paraffin-embedded lung cancer tissues (left) and colon cancer tissues (right) using CDC27 mouse mAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.