CDC27 Primary Antibody

Item Information
Catalog #Size/ConcentrationPrice
Specification
AliasesAPC3; HNUC; ANAPC3; CDC27Hs; D0S1430E; D17S978E;
ProductOrderC
Clone#5C12
Entrez GenelD996
FormulationAscitic fluid containing 0.03% sodium azide.
HostMouse
IsotypeIgG1
ImmunogenPurified recombinant fragment of human CDC27 expressed in E. Coli.
MW91kDa
Shipping InformationThis product will ship in a box containing blue ice at a temperature of 4°C. Learn More
Species ReactivityHuman
Application
ELISA1/10000
IHC_P(Immunohistochemistry)1/200 - 1/1000
WB (Western Blot)1/500 - 1/2000
Sequence
724-830
Catalog#: 30064
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Images
Western Blot
Figure 1: Western blot analysis using CDC27 mouse mAb against CDC27(AA: 724-830)-hIgGFc transfected HEK293 cell.
Figure 1: Western blot analysis using CDC27 mouse mAb against CDC27(AA: 724-830)-hIgGFc transfected HEK293 cell.
Immunohistochemical analysis
Figure 2: Immunohistochemical analysis of paraffin-embedded lung cancer tissues (left) and colon cancer tissues (right) using CDC27 mouse mAb with DAB staining.
Figure 2: Immunohistochemical analysis of paraffin-embedded lung cancer tissues (left) and colon cancer tissues (right) using CDC27 mouse mAb with DAB staining.
Product Overview
Description

Cdc27 shares strong similarity with Saccharomyces cerevisiae protein Cdc27, and the gene product of Schizosaccharomyces pombe nuc 2. It is a component of the Anaphase Promoting Complex (APC), which is composed of eight protein subunits and is highly conserved in eucaryotic cells. The APC catalyzes the formation of the cyclin B ubiquitin conjugate that is responsible for the ubiquitin mediated proteolysis of B type cyclins. This protein and 3 other members of the APC complex contain the TPR (tetratricopeptide repeat), a protein domain important for protein protein interaction. This protein was shown to interact with mitotic checkpoint proteins including Mad2, p55CDC and BUBR1, and thus may be involved in controlling the timing of mitosis.

References (references)
References (references)1. Mol Cell Biol. 2004 May;24(9):3577-87.
2. J Biol Chem. 2004 Oct 1;279(40):42128-38.