CDC2 Primary Antibody

Item Information
Catalog #Size/ConcentrationPrice
Specification
AliasesCDC2; CDC28A; P34CDC2; MGC111195; DKFZp686L20222; CDK1
ProductOrderC
Clone#8C5A6
Entrez GenelD983
FormulationAscitic fluid containing 0.03% sodium azide.
HostMouse
IsotypeIgG1
ImmunogenPurified recombinant fragment of CDC2 expressed in E. Coli.
MW34kDa
Shipping InformationThis product will ship in a box containing blue ice at a temperature of 4°C. Learn More
Species ReactivityHuman
Application
ELISA1/10000
IHC_P(Immunohistochemistry)1/200 - 1/1000
WB (Western Blot)1/500 - 1/2000
Sequence
full(1-297)
Catalog#: 30038
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Images
Western Blot
Figure 1: Western blot analysis using CDC2 mouse mAb against Jurkat (1) cell lysate.
Figure 1: Western blot analysis using CDC2 mouse mAb against Jurkat (1) cell lysate.
Immunohistochemical analysis
Figure 2: Immunohistochemical analysis of paraffin-embedded human lung cancer (A) and esophageal cancer (B), showing cytoplasmic localization using CDC2 mouse mAb with DAB staining.
Figure 2: Immunohistochemical analysis of paraffin-embedded human lung cancer (A) and esophageal cancer (B), showing cytoplasmic localization using CDC2 mouse mAb with DAB staining.
Product Overview
Description

The cell division control protein cdc2, also known as cyclin-dependent kinase 1 (Cdk1) or p34/cdk1, plays a key role in the control of the eukaryotic cell cycle, where it is required for entry into S-phase and mitosis. Cdc2 exists as a complex with both cyclin A and cyclin B. The best characterized of these associations is the Cdc2 p34 cyclin B complex, which is required for the G2 to M phase transition. Activation of Cdc2 is controlled at several steps including cyclin binding and phosphorylation of threonine 161. However, the critical regulatory step in activating cdc2 during progression into mitosis appears to be dephosphorylation of Tyr15 and Tyr14. Phosphorylation at Tyr15 and inhibition of Cdc2 is carried out by WEE1 and MIK protein kinases while Tyr15 dephosphorylation and activation of Cdc2 is carried out by the cdc25 phosphatase. The isoform CDC2deltaT is found in breast cancer tissues. Furthermore, cdc2/Cdk1 is a key mediator of neuronal cell death in brain development and degeneration.

References (references)
References (references)1. Mol Biol Cell. 2008 Aug;19(8):3536-43.
2. Eur J Cancer. 2009 May;45(8):1466-73.