CD8A Primary Antibody
Item Information
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Size
Price
Description
The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen acts as a coreceptor with the T-cell receptor on the T lymphocyte to recognize antigens displayed by an antigen presenting cell in the context of class I MHC molecules. The coreceptor functions as either a homodimer composed of two alpha chains or as a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene encodes the CD8 alpha chain. Multiple transcript variants encoding different isoforms have been found for this gene.
Product Overview
Entrez GenelD
925
Aliases
CD8; MAL; p32; Leu2
Clone#
6A9A5
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human CD8A (AA: extra 22-182) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.J Clin Immunol. 2015 Nov;35(8):692-5.2.Blood Transfus. 2014 Jan;12 Suppl 1:s105-8.
Product Image
Elisa
Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using CD8A mAb against human CD8A (AA: extra 22-182) recombinant protein. (Expected MW is *** kDa)
Western Blot
Figure 3:Western blot analysis using CD8A mAb against HEK293 (1) and CD8A (AA: extra 22-182)-hIgGFc transfected HEK293 (2) cell lysate.
Flow cytometric
Figure 4:Flow cytometric analysis of HL-60 cells using CD8A mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.