CD8a Primary Antibody
The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen acts as a coreceptor with the T-cell receptor on the T lymphocyte to recognize antigens displayed by an antigen presenting cell in the context of class I MHC molecules. The coreceptor functions as either a homodimer composed of two alpha chains or as a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene encodes the CD8 alpha chain. Multiple transcript variants encoding different isoforms have been found for this gene.
Purified antibody in PBS with 0.05% sodium azide
4°C; -20°C for long term storage
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 2:Western blot analysis using CD8a mAb against human CD8a (AA: 22–235) recombinant protein. (Expected MW is 26.4 kDa)
Figure 3:Western blot analysis using CD8a mAb against HEK293 (1) and CD8a (AA: 22–235)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 4:Western blot analysis using CD8a mouse mAb against K562 (1), Jurkat (2), and HL-60 (3) cell lysate.
Figure 5:Flow cytometric analysis of Hela cells using CD8a mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.