CD57 Primary Antibody
Item Information
Catalog #
Size
Price
Description
The protein encoded by this gene is a member of the glucuronyltransferase gene family. These enzymes exhibit strict acceptor specificity, recognizing nonreducing terminal sugars and their anomeric linkages. This gene product functions as the key enzyme in a glucuronyl transfer reaction during the biosynthesis of the carbohydrate epitope HNK-1 (human natural killer-1, also known as CD57 and LEU7). Alternate transcriptional splice variants have been characterized. [provided by RefSeq, Jul 2008]
Product Overview
Entrez GenelD
27087
Aliases
NK1; CD57; HNK1; LEU7; NK-1; GLCATP; GLCUATP
Clone#
1A4F10
Host / Isotype
Mouse / Mouse IgG2b
Immunogen
Purified recombinant fragment of human CD57 (AA: 28-334) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200-1/1000
FCM (Flow Cytometry)
1/200-1/400
ELISA
1/10000
References
1,Mol Neurobiol. 2019 Sep;56(9):6581-6585. 2,J Acquir Immune Defic Syndr. 2016 Jun 1;72(2):133-7.
Product Image
ELISA
![Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/graph-elisa_31967ea_91.jpg?v=1666705052)
Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng)
WESTERN BLOT
![Figure 2: Western blot analysis using CD57 mAb against human CD57 (AA: 28-334) recombinant protein. (Expected MW is 37.9kDa)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/graph-wb_31967rwb_92.jpg?v=1666705052)
Figure 2: Western blot analysis using CD57 mAb against human CD57 (AA: 28-334) recombinant protein. (Expected MW is 37.9kDa)
WESTERN BLOT
![Figure 3: Western blot analysis using CD57 mAb against HEK293-6e (1) and CD57 (AA: 28-334)-hIgGFc transfected HEK293-6e (2) cell lysate.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/graph-wb_31967twb_93.jpg?v=1666705052)
Figure 3: Western blot analysis using CD57 mAb against HEK293-6e (1) and CD57 (AA: 28-334)-hIgGFc transfected HEK293-6e (2) cell lysate.
FLOW CYTOMETRY
![Figure 4: Flow cytometric analysis of Jurkat cells using CD57 mouse mAb (green) and negative control (red).](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/graph-fcm_31967fcm_94.jpg?v=1666705052)
Figure 4: Flow cytometric analysis of Jurkat cells using CD57 mouse mAb (green) and negative control (red).
FLOW CYTOMETRY
![Figure 5: Flow cytometric analysis of Hela cells using CD57 mouse mAb (green) and negative control (red).](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/graph-fcm_31967fcm-2_95.jpg?v=1666705052)
Figure 5: Flow cytometric analysis of Hela cells using CD57 mouse mAb (green) and negative control (red).
IMMUNOHISTOCHEMISTRY
![Figure 6: Immunohistochemical analysis of paraffin-embedded brain tissues using CD57 mouse mAb with DAB staining.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/graph-icc_31967ihc_96.jpg?v=1666705052)
Figure 6: Immunohistochemical analysis of paraffin-embedded brain tissues using CD57 mouse mAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.