CD57 Primary Antibody

Item Information

Catalog #

Size

Price

Description

The protein encoded by this gene is a member of the glucuronyltransferase gene family. These enzymes exhibit strict acceptor specificity, recognizing nonreducing terminal sugars and their anomeric linkages. This gene product functions as the key enzyme in a glucuronyl transfer reaction during the biosynthesis of the carbohydrate epitope HNK-1 (human natural killer-1, also known as CD57 and LEU7). Alternate transcriptional splice variants have been characterized.

Product Overview

Entrez GenelD

27087

Aliases

B3GAT1; NK1; HNK1; LEU7; NK-1; GLCATP; GLCUATP

Clone#

3F6D6

Host / Isotype

Mouse / IgG1

Species Reactivity

Human, Mouse, Rat, Monkey

Immunogen

Purified recombinant fragment of human CD57 (AA: 35-191) expressed in E. Coli.

Formulation

Purified antibody in PBS with 0.05% sodium azide

Storage

4°C; -20°C for long term storage

Product Applications

WB (Western Blot)

1/500 - 1/2000

FCM (Flow Cytometry)

1/200 - 1/400

ELISA

1/10000

References

1. Anticancer Res. 2014 Oct;34(10):5729-34.
2. Biomed Res Int. 2014;2014:356427.

Product Image

Elisa

Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Western Blot

Figure 2:Western blot analysis using CD57 mAb against human CD57 (AA: 35-191) recombinant protein. (Expected MW is 44 kDa)

Western Blot

Figure 3:Western blot analysis using CD57 mAb against HEK293 (1) and CD57 (AA: 35-191)-hIgGFc transfected HEK293 (2) cell lysate.

Western Blot

Figure 4:Western blot analysis using CD57 mouse mAb against SK-N-SH (1), SH-SY5Y (2), C6 (3), HL-60 (4), COS7 (5), Hela (6), and NIH/3T3 (7) cell lysate.

Flow cytometric