CD55 Primary Antibody
Item Information
Catalog #
Size
Price
Description
This gene encodes a protein involved in the regulation of the complement cascade. The encoded glycoprotein is also known as the decay-accelerating factor (DAF); binding of DAF to complement proteins accelerates their decay, disrupting the cascade and preventing damage to host cells. Antigens present on the DAF glycoprotein constitute the Cromer blood group system (CROM). Two alternatively spliced transcripts encoding different proteins have been identified. The predominant transcript encodes a membrane-bound protein expressed on cells exposed to plasma component proteins but an alternatively spliced transcript produces a soluble protein present at much lower levels. Additional, alternatively spliced transcript variants have been described, but their biological validity has not been determined.
Product Overview
Entrez GenelD
1604
Aliases
CR; TC; DAF; CROM
Clone#
3F12
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human CD55 (AA: 238-357) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Product Applications
WB (Western Blot)
1/500 - 1/2000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
Propose dilution 1/10000
References
1.Indian J Med Res. 2011 Jun;133:662-4.
2.Cell Immunol. 2010;265(2):127-32.
2.Cell Immunol. 2010;265(2):127-32.
Product Image
Western Blot
Figure 1: Western blot analysis using CD55 mAb against human CD55 recombinant protein. (Expected MW is 38.5 kDa)
Western Blot
Figure 2: Western blot analysis using CD55 mouse mAb against Raji (1) and K562 (2) cell lysate.
Flow cytometric
Figure 3: Flow cytometric analysis of K562 cells using CD55 mouse mAb (green) and negative control (red).
Elisa
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
For Research Use Only. Not for use in diagnostic procedures.