CD54 Primary Antibody

Item Information
Catalog #
Size
Price
Description

This gene encodes a cell surface glycoprotein which is typically expressed on endothelial cells and cells of the immune system. It binds to integrins of type CD11a / CD18, or CD11b / CD18 and is also exploited by Rhinovirus as a receptor.

Product Overview
Entrez GenelD
3383
Aliases
ICAM1; BB2; P3.58
Clone#
3A5G12
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human CD54 (AA: extra 28-163) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Oxid Med Cell Longev. 2016;2016:1852340.2.Cell Microbiol. 2016 Aug;18(8):1043-55.
Product Image
Elisa
Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using CD54 mAb against human CD54 (AA: extra 28-163) recombinant protein. (Expected MW is 40.8 kDa)
Figure 2:Western blot analysis using CD54 mAb against human CD54 (AA: extra 28-163) recombinant protein. (Expected MW is 40.8 kDa)
Western Blot
Figure 3:Western blot analysis using CD54 mAb against HEK293 (1) and CD54 (AA: extra 28-163)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 3:Western blot analysis using CD54 mAb against HEK293 (1) and CD54 (AA: extra 28-163)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot
Figure 4:Western blot analysis using CD54 mouse mAb against C6 (1) and HUVEC (2) cell lysate.
Figure 4:Western blot analysis using CD54 mouse mAb against C6 (1) and HUVEC (2) cell lysate.
Flow cytometric
Figure 5:Flow cytometric analysis of HL-60 cells using CD54 mouse mAb (green) and negative control (red).
Figure 5:Flow cytometric analysis of HL-60 cells using CD54 mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.