CD5 Primary Antibody
Item Information
Catalog #
Size
Price
Description
The human cd5 gene has two alternative exons 1: exon 1A (E1A) which encodes the full-length (FL) CD5 protein and exon 1B (E1B) which encodes a truncated (TR) isoform. The FL variant of CD5 protein is translocated to the plasma membrane, while its TR variant is retained in the cytoplasm.
Product Overview
Entrez GenelD
921
Aliases
T1; LEU1
Clone#
6A11
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human CD5 (AA: 27-233) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Product Applications
WB (Western Blot)
1/250
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Crit Rev Immunol. 2011;31(1):31-42. 2.Blood. 2011 Dec 1;118(23):6107-14.
Product Image
Western Blot
Figure 1: Western blot analysis using CD5 mAb against human CD5 recombinant protein. (Expected MW is 48.3 kDa)
Western Blot
Figure 2: Western blot analysis using CD5 mouse mAb against K562 (1), Jurkat (2), Raji (3), and MOLT4 (4) cell lysate.
Flow cytometric
Figure 3: Flow cytometric analysis of Jurkat cells using CD5 mouse mAb (green) and negative control (red).
Elisa
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
For Research Use Only. Not for use in diagnostic procedures.