CD5 Primary Antibody

Item Information
Catalog #Size/ConcentrationPrice
Specification
AliasesT1; LEU1
ProductOrderC
Clone#6A11
Entrez GenelD921
FormulationPurified antibody in PBS with 0.05% sodium azide
HostMouse
IsotypeIgG1
ImmunogenPurified recombinant fragment of human CD5 (AA: 27-233) expressed in E. Coli.
MW54.6kDa
Shipping InformationThis product will ship in a box containing blue ice at a temperature of 4°C. Learn More
Species ReactivityHuman
Application
ELISA1/10000
FCM (Flow Cytometry)1/200 - 1/400
WB (Western Blot)1/250
Sequence
27-233
Catalog#: 30400
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Images
Western Blot
Figure 1: Western blot analysis using CD5 mAb against human CD5 recombinant protein. (Expected MW is 48.3 kDa)
Figure 1: Western blot analysis using CD5 mAb against human CD5 recombinant protein. (Expected MW is 48.3 kDa)
Western Blot
Figure 2: Western blot analysis using CD5 mouse mAb against K562 (1), Jurkat (2), Raji (3), and MOLT4 (4) cell lysate.
Figure 2: Western blot analysis using CD5 mouse mAb against K562 (1), Jurkat (2), Raji (3), and MOLT4 (4) cell lysate.
Flow cytometric
Figure 3: Flow cytometric analysis of Jurkat cells using CD5 mouse mAb (green) and negative control (red).
Figure 3: Flow cytometric analysis of Jurkat cells using CD5 mouse mAb (green) and negative control (red).
Elisa
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Product Overview
Description

The human cd5 gene has two alternative exons 1: exon 1A (E1A) which encodes the full-length (FL) CD5 protein and exon 1B (E1B) which encodes a truncated (TR) isoform. The FL variant of CD5 protein is translocated to the plasma membrane, while its TR variant is retained in the cytoplasm.

References (references)
References (references)1.Crit Rev Immunol. 2011;31(1):31-42. 2.Blood. 2011 Dec 1;118(23):6107-14.