CD49E Primary Antibody
Item Information
Catalog #
Size
Price
Description
The product of this gene belongs to the integrin alpha chain family. Integrins are heterodimeric integral membrane proteins composed of an alpha subunit and a beta subunit that function in cell surface adhesion and signaling. The encoded preproprotein is proteolytically processed to generate light and heavy chains that comprise the alpha 5 subunit. This subunit associates with the beta 1 subunit to form a fibronectin receptor. This integrin may promote tumor invasion, and higher expression of this gene may be correlated with shorter survival time in lung cancer patients. Note that the integrin alpha 5 and integrin alpha V subunits are encoded by distinct genes.
Product Overview
Entrez GenelD
3678
Aliases
ITGA5; FNRA; VLA-5; VLA5A
Clone#
6F6C1
Host / Isotype
Mouse / IgG2a
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human CD49E (AA: extra 111-253) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Product Applications
WB (Western Blot)
1/500 - 1/2000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Int J Clin Exp Pathol. 2015 Nov 1;8(11):14425-32.
2.Cancer Biomark. 2013;13(5):323-8.
2.Cancer Biomark. 2013;13(5):323-8.
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using CD49E mAb against human CD49E (AA: extra 111-253) recombinant protein. (Expected MW is 41.8 kDa)
Western Blot
Figure 3:Western blot analysis using CD49E mAb against HEK293 (1) and CD49E (AA: extra 111-253)-hIgGFc transfected HEK293 (2) cell lysate.
Flow cytometric
Figure 4:Flow cytometric analysis of HL-60 cells using CD49E mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.