CD49B Primary Antibody

Item Information

Catalog #

Size

Price

Description

This gene encodes the alpha subunit of a transmembrane receptor for collagens and related proteins. The encoded protein forms a heterodimer with a beta subunit and mediates the adhesion of platelets and other cell types to the extracellular matrix. Loss of the encoded protein is associated with bleeding disorder platelet-type 9. Antibodies against this protein are found in several immune disorders, including neonatal alloimmune thrombocytopenia. This gene is located adjacent to a related alpha subunit gene. Alternative splicing results in multiple transcript variants.

Product Overview

Entrez GenelD

3673

Aliases

ITGA2; BR; GPIa; HPA-5; VLA-2; VLAA2

Clone#

5E3A8

Host / Isotype

Mouse / IgG1

Species Reactivity

Human

Immunogen

Purified recombinant fragment of human CD49B (AA: extra 994-1132) expressed in E. Coli.

Formulation

Purified antibody in PBS with 0.05% sodium azide

Storage

4°C; -20°C for long term storage

Product Applications

WB (Western Blot)

1/500 - 1/2000

ICC (Immunocytochemistry)

1/100 - 1/500

FCM (Flow Cytometry)

1/200 - 1/400

ELISA

1/10000

References

1.Arch Oral Biol. 2014 Mar;59(3):283-8.
2.Int J Clin Exp Pathol. 2013 Apr 15;6(5):841-52.

Product Image

Elisa

Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Western Blot

Figure 2:Western blot analysis using CD49B mAb against human CD49B (AA: extra 994-1132) recombinant protein. (Expected MW is 41.5 kDa)

Western Blot

Figure 3:Western blot analysis using CD49B mAb against HEK293 (1) and CD49B (AA: extra 994-1132)-hIgGFc transfected HEK293 (2) cell lysate.

Immunofluorescence analysis

Figure 4:Immunofluorescence analysis of Hela cells using CD49B mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Flow cytometric