TIGIT Primary Antibody
Item Information
Catalog #
Size
Price
Description
This gene encodes a member of the PVR (poliovirus receptor) family of immunoglobin proteins. The product of this gene is expressed on several classes of T cells including follicular B helper T cells (TFH). The protein has been shown to bind PVR with high affinity; this binding is thought to assist interactions between TFH and dendritic cells to regulate T cell dependent B cell responses.
Product Overview
Entrez GenelD
201633
Aliases
VSIG9; VSTM3; WUCAM
Clone#
4C5B9
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human TIGIT (AA: extra 22-141) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Product Applications
WB (Western Blot)
1/500 - 1/2000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Clin Cancer Res. 2016 Jun 15;22(12):3057-66.
2.Eur J Immunol. 2015 Oct;45(10):2886-97.
2.Eur J Immunol. 2015 Oct;45(10):2886-97.
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using TIGIT mAb against human TIGIT (AA: extra 22-141) recombinant protein. (Expected MW is 39 kDa)
Western Blot
Figure 3:Western blot analysis using TIGIT mAb against HEK293 (1) and TIGIT (AA: extra 22-141)-hIgGFc transfected HEK293 (2) cell lysate.
Flow cytometric
Figure 4:Flow cytometric analysis of K562 cells using TIGIT mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.