CD3E Primary Antibody
Item Information
Catalog #
Size
Price
Description
The protein encoded by this gene is the CD3-epsilon polypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. Defects in this gene cause immunodeficiency. This gene has also been linked to a susceptibility to type I diabetes in women.
Product Overview
Entrez GenelD
916
Aliases
T3E; TCRE; FLJ18683; CD3E
Clone#
4E2
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of CD3E expressed in E. Coli.
Formulation
Ascitic fluid containing 0.03% sodium azide.
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Product Applications
WB (Western Blot)
1/500 - 1/2000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1. Nat Methods. 2005 Aug;2(8):591-8.
2. J Cell Physiol. 2006 Dec;209(3):695-700. Review.
3. J Biol Chem. 2006 Dec 1;281(48):36977-84. Epub 2006 Oct 5.
2. J Cell Physiol. 2006 Dec;209(3):695-700. Review.
3. J Biol Chem. 2006 Dec 1;281(48):36977-84. Epub 2006 Oct 5.
Product Image
Western Blot
Figure 1: Western blot analysis using CD3E mouse mAb against Jurkat (1) cell lysate.
Flow cytometric
Figure 2: Flow cytometric analysis of Jurkat cells using CD3E mouse mAb (green) and negative control (purple).
For Research Use Only. Not for use in diagnostic procedures.