CD3D Primary Antibody
The protein encoded by this gene is part of the T-cell receptor/CD3 complex (TCR/CD3 complex) and is involved in T-cell development and signal transduction. The encoded membrane protein represents the delta subunit of the CD3 complex, and along with four other CD3 subunits, binds either TCR alpha/beta or TCR gamma/delta to form the TCR/CD3 complex on the surface of T-cells. Defects in this gene are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (SCIDBNK). Two transcript variants encoding different isoforms have been found for this gene. Other variants may also exist, but the full-length natures of their transcripts has yet to be defined. [provided by RefSeq, Feb 2009]
![Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/graph-elisa_31926ea_418.jpg?v=1666704931)
![Figure 2: Western blot analysis using CD3D mAb against human CD3D (AA: 127-171) recombinant protein. (Expected MW is 18.6 kDa)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/graph-wb_31926rwb_419.jpg?v=1666704931)
![Figure 3: Western blot analysis using CD3D mAb against HEK293-6e (1) and CD3D (AA: 127-171))-hIgGFc transfected HEK293-6e (2) cell lysate.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/graph-wb_31926twb_420.jpg?v=1666704931)
![Figure 4: Flow cytometric analysis of Jurkat cells using CD3D mouse mAb (green) and negative control (red).](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/graph-fcm_31926fcm_421.jpg?v=1666704931)
![Figure 5: Flow cytometric analysis of THP-1 cells using CD3D mouse mAb (green) and negative control (red).](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/graph-fcm_31926fcm-2_422.jpg?v=1666704931)
![Figure 6: Immunofluorescence analysis of Hela cells using CD3D mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/graph-icc_31926icc_423.jpg?v=1666704931)