CD370 Primary Antibody
Item Information
Catalog #
Size
Price
Description
CLEC9A is a group V C-type lectin-like receptor (CTLR) that functions as an activation receptor and is expressed on myeloid lineage cells.
Product Overview
Entrez GenelD
283420
Aliases
CLEC9A; DNGR1; DNGR-1; UNQ9341
Clone#
2H6H10
Host / Isotype
Mouse / IgG2b
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human CD370 (AA: extra 57-241) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
ICC (Immunocytochemistry)
1/200
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Semin Cell Dev Biol. 2015 May;41:39-48.
2.Blood. 2012 Mar 8;119(10):2284-92.
2.Blood. 2012 Mar 8;119(10):2284-92.
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using CD370 mAb against human CD370 (AA: extra 57-241) recombinant protein. (Expected MW is 47.2 kDa)
Western Blot
Figure 3:Western blot analysis using CD370 mAb against HEK293 (1) and CD370 (AA: extra 57-241)-hIgGFc transfected HEK293 (2) cell lysate.
Immunofluorescence analysis
Figure 4:Immunofluorescence analysis of Hela cells using CD370 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric
Figure 5:Flow cytometric analysis of HL-60 cells using CD370 mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.