CD369 Primary Antibody

Item Information
Catalog #
Size
Price
Description

This gene encodes a member of the C-type lectin/C-type lectin-like domain (CTL/CTLD) superfamily. The encoded glycoprotein is a small type II membrane receptor with an extracellular C-type lectin-like domain fold and a cytoplasmic domain with an immunoreceptor tyrosine-based activation motif. It functions as a pattern-recognition receptor that recognizes a variety of beta-1,3-linked and beta-1,6-linked glucans from fungi and plants, and in this way plays a role in innate immune response. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. This gene is closely linked to other CTL/CTLD superfamily members on chromosome 12p13 in the natural killer gene complex region.

Product Overview
Entrez GenelD
64581
Aliases
CLEC7A; BGR; CANDF4; SCARE2; DECTIN1; CLECSF12
Clone#
4G2G6
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human CD369 (AA: extra 66-247) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
ICC (Immunocytochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Int Immunopharmacol. 2017 Nov;52:168-175.
2.Med Mycol. 2017 Jul 1;55(5):573-578.
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using CD369 mAb against human CD369 (AA: extra 66-247) recombinant protein. (Expected MW is 46.5 kDa)
Figure 2:Western blot analysis using CD369 mAb against human CD369 (AA: extra 66-247) recombinant protein. (Expected MW is 46.5 kDa)
Western Blot
Figure 3:Western blot analysis using CD369 mAb against HEK293 (1) and CD369 (AA: extra 66-247)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 3:Western blot analysis using CD369 mAb against HEK293 (1) and CD369 (AA: extra 66-247)-hIgGFc transfected HEK293 (2) cell lysate.
Flow cytometric
Figure 4:Flow cytometric analysis of HL-60 cells using CD369 mouse mAb (green) and negative control (red).
Figure 4:Flow cytometric analysis of HL-60 cells using CD369 mouse mAb (green) and negative control (red).
Immunofluorescence analysis
Figure 5:Immunofluorescence analysis of Hela cells using CD369 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Figure 5:Immunofluorescence analysis of Hela cells using CD369 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
For Research Use Only. Not for use in diagnostic procedures.