Mouse Monoclonal Antibody to CD360

Item Information
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Description

The protein encoded by this gene is a cytokine receptor for interleukin 21 (IL21). It belongs to the type I cytokine receptors, and has been shown to form a heterodimeric receptor complex with the common gamma-chain, a receptor subunit also shared by the receptors for interleukin 2, 4, 7, 9, and 15. This receptor transduces the growth promoting signal of IL21, and is important for the proliferation and differentiation of T cells, B cells, and natural killer (NK) cells. The ligand binding of this receptor leads to the activation of multiple downstream signaling molecules, including JAK1, JAK3, STAT1, and STAT3. Knockout studies of a similar gene in mouse suggest a role for this gene in regulating immunoglobulin production. Three alternatively spliced transcript variants have been described.

Product Overview
Entrez GenelD
50615
Aliases
IL21R; NILR; IMD56
Clone#
1C3G9
Host / Isotype
Mouse / IgG1
Immunogen
Purified recombinant fragment of human CD360 (AA: extra 20-232) expressed in HEK293-6e cells supernatant
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4℃; -20℃ for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
ICC (Immunocytochemistry)
1/25 - 1/200
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.J Clin Lab Anal. 2019 Jun;33(5):e22860. 2.Haematologica. 2017 Apr;102(4):773-784.
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using CD360 mAb against human CD360 (AA: extra 20-232) recombinant protein. (Expected MW is 55 kDa)
Figure 2:Western blot analysis using CD360 mAb against human CD360 (AA: extra 20-232) recombinant protein. (Expected MW is 55 kDa)
Immunofluorescence analysis
Figure 3:Immunofluorescence analysis of Hela cells using CD360 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Figure 3:Immunofluorescence analysis of Hela cells using CD360 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric analysis
Figure 4:Flow cytometric analysis of K562 cells using CD360 mouse mAb (green) and negative control (red).
Figure 4:Flow cytometric analysis of K562 cells using CD360 mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.