CD354 Primary Antibody

Item Information
Catalog #
Size
Price
Description

This gene encodes a receptor belonging to the Ig superfamily that is expressed on myeloid cells. This protein amplifies neutrophil and monocyte-mediated inflammatory responses triggered by bacterial and fungal infections by stimulating release of pro-inflammatory chemokines and cytokines, as well as increased surface expression of cell activation markers. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene.

Product Overview
Entrez GenelD
54210
Aliases
TREM1; TREM-1
Clone#
6B12D1
Host / Isotype
Mouse / IgG1
Species Reactivity
Human, Rat, Monkey
Immunogen
Purified recombinant fragment of human CD354 (AA: extra 21-205) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Mol Oral Microbiol. 2017 Aug;32(4):275-287.
2.Immunol Lett. 2016 Mar;171:50-9.
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using CD354 mAb against human CD354 (AA: extra 21-205) recombinant protein. (Expected MW is 46.8 kDa)
Figure 2:Western blot analysis using CD354 mAb against human CD354 (AA: extra 21-205) recombinant protein. (Expected MW is 46.8 kDa)
Western Blot
Figure 3:Western blot analysis using CD354 mAb against HEK293 (1) and CD354 (AA: extra 21-205)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 3:Western blot analysis using CD354 mAb against HEK293 (1) and CD354 (AA: extra 21-205)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot
Figure 4:Western blot analysis using CD354 mouse mAb against HepG2 (1), COS7 (2), A549 (3), SPC-A-1 (4), and C6 (5) cell lysate.
Figure 4:Western blot analysis using CD354 mouse mAb against HepG2 (1), COS7 (2), A549 (3), SPC-A-1 (4), and C6 (5) cell lysate.
Flow cytometric
Figure 5:Flow cytometric analysis of HL-60 cells using CD354 mouse mAb (green) and negative control (red).
Figure 5:Flow cytometric analysis of HL-60 cells using CD354 mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.