Mouse Monoclonal Antibody to CD35
Item Information
Catalog #
Size
Price
Description
This gene encodes a glycosyl phosphatidylinositol (GPI)-linked glycoprotein that localizes to the surface of platelets, activated T-cells, and endothelial cells. The protein binds to and negatively regulates signalling by transforming growth factor beta (TGF-beta). Multiple transcript variants encoding different isoforms have been found for this gene.
Product Overview
Entrez GenelD
135228
Aliases
p180; r150; CPAMD7
Clone#
1D4H7
Host / Isotype
Mouse / IgG1
Immunogen
Purified recombinant fragment of human CD35 (AA: extra 1274-1421) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4℃; -20℃ for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.J Huazhong Univ Sci Technolog Med Sci. 2016 Feb;36(1):132-136. 2.Diagn Pathol. 2015 Aug 7;10:137.
Product Image
Elisa
![Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/30ee5618a675398045cd339e91572543e1aa925cb828ac7df837e880044fc924.jpg?v=1666705862)
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
![Figure 2:Western blot analysis using CD35 mAb against human CD35 (AA: extra 1274-1421) recombinant protein. (Expected MW is 42.6 kDa)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/8fe894fc84c07ecbbb9cc12b940d6881ba5ec212c95599fc8e8d00c55cd7ef7e.jpg?v=1666705862)
Figure 2:Western blot analysis using CD35 mAb against human CD35 (AA: extra 1274-1421) recombinant protein. (Expected MW is 42.6 kDa)
Western Blot
![Figure 3:Western blot analysis using CD35 mAb against HEK293-6e (1) and CD35 (AA: extra 1274-1421)-hIgGFc transfected HEK293-6e (2) cell lysate.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/483b2db01851467f7ec18d9f4fa96e4a6f1228f9e47dd53003c97285fa2f25f1.jpg?v=1666705862)
Figure 3:Western blot analysis using CD35 mAb against HEK293-6e (1) and CD35 (AA: extra 1274-1421)-hIgGFc transfected HEK293-6e (2) cell lysate.
Flow cytometric analysis
![Figure 4:Flow cytometric analysis of THP-1 cells using CD35 mouse mAb (green) and negative control (red).](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/f8b27d1770b8ae681bd13c39a3f656f25bd2061a028dff26eef374104feeca0f.jpg?v=1666705862)
Figure 4:Flow cytometric analysis of THP-1 cells using CD35 mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.