Mouse Monoclonal Antibody to CD34
Item Information
Catalog #
Size
Price
Description
The protein encoded by this gene may play a role in the attachment of stem cells to the bone marrow extracellular matrix or to stromal cells. This single-pass membrane protein is highly glycosylated and phosphorylated by protein kinase C. Two transcript variants encoding different isoforms have been found for this gene.
Product Overview
Entrez GenelD
947
Aliases
undefined
Clone#
6C7G7
Host / Isotype
Mouse / IgG1
Immunogen
Purified recombinant fragment of human CD34 (AA: extra 32-290) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4℃; -20℃ for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Stem Cell Res Ther. 2019 May 21;10(1):147. 2.Circ Res. 2017 Apr 28;120(9):1466-1476.
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using CD34 mAb against human CD34 (AA: extra 32-290) recombinant protein. (Expected MW is 53.3 kDa)
Western Blot
Figure 3:Western blot analysis using CD34 mAb against HEK293-6e (1) and CD34 (AA: extra 32-290)-hIgGFc transfected HEK293-6e (2) cell lysate.
Flow cytometric analysis
Figure 4:Flow cytometric analysis of THP-1 cells using CD34 mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 5:Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using CD34 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 6:Immunohistochemical analysis of paraffin-embedded muscle tissues using CD34 mouse mAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.