CD34 Primary Antibody
Item Information
Catalog #
Size
Price
Description
The protein encoded by this gene may play a role in the attachment of stem cells to the bone marrow extracellular matrix or to stromal cells. This single-pass membrane protein is highly glycosylated and phosphorylated by protein kinase C. Two transcript variants encoding different isoforms have been found for this gene.
Product Overview
Entrez GenelD
947
Clone#
1D3H10
Host / Isotype
Mouse / Mouse IgG2b
Immunogen
Purified recombinant fragment of human CD34 (AA: extra 32-290) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
FCM (Flow Cytometry)
1/200-1/400
ELISA
1/10000
References
1.Stem Cell Res Ther. 2019 May 21;10(1):147. 2.Circ Res. 2017 Apr 28;120(9):1466-1476.
Product Image
ELISA
Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng)
WESTERN BLOT
Figure 2: Western blot analysis using CD34 mAb against human CD34 (AA: extra 32-290) recombinant protein. (Expected MW is 53.3 kDa)
WESTERN BLOT
Figure 3: Western blot analysis using CD34 mAb against HEK293-6e (1) and CD34 (AA: extra 32-290)-hIgGFc transfected HEK293-6e (2) cell lysate.
WESTERN BLOT
Figure 4: Western blot analysis using CD34 mouse mAb against mouse lung (1), rat lung (2), mouse kidney (3), and rat kidney (4) tissue lysate.
FLOW CYTOMETRY
Figure 5: Flow cytometric analysis of THP-1 cells using CD34 mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.