CD34 Primary Antibody
Item Information
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Price
Description
CD34 is a transmembrane glycoprotein with a molecular mass of approximately 110 kD that is selectively expressed on human hematopoietic progenitor cells, endothelial cells and some fibroblasts. It could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. CD34 is highly expressed on hematopoietic progenitors, as well as on endothelial cells. CD34 has been used to measure angiogenesis, which reportedly predicts tumor recurrence.
Product Overview
Entrez GenelD
947
Aliases
CD34
Clone#
4H5E7
Host / Isotype
Mouse / IgG2b
Species Reactivity
Human
Immunogen
Purified recombinant fragment of CD34 expressed in E. Coli.
Formulation
Ascitic fluid containing 0.03% sodium azide.
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
ICC (Immunocytochemistry)
1/200 - 1/1000
ELISA
1/10000
References
1. Sho E., et al. Arterioscler Thromb Vasc Biol. 2004: 24: 1916-21.
2. Satterthwaite, A.B.,et al. Genomics.1992:12:788-794.
3. Krause, D.S.,et al. 1994. Blood.84: 691-701.
2. Satterthwaite, A.B.,et al. Genomics.1992:12:788-794.
3. Krause, D.S.,et al. 1994. Blood.84: 691-701.
Product Image
Western Blot
Figure 1: Western blot analysis using CD34 mouse mAb against truncated CD34 recombinant protein.
Immunohistochemical analysis
Figure 2: Immunohistochemical analysis of paraffin-embedded human spleen using CD34 mouse mAb with DAB staining.
Immunofluorescence analysis
Figure 3: Immunofluorescence analysis of peripheral blood cells using CD34 mouse mAb.
For Research Use Only. Not for use in diagnostic procedures.