Mouse Monoclonal Antibody to CD338

Item Information

Catalog #

Size

Price

Description

The membrane-associated protein encoded by this gene is included in the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the White subfamily. Alternatively referred to as a breast cancer resistance protein, this protein functions as a xenobiotic transporter which may play a major role in multi-drug resistance. It likely serves as a cellular defense mechanism in response to mitoxantrone and anthracycline exposure. Significant expression of this protein has been observed in the placenta, which may suggest a potential role for this molecule in placenta tissue. Multiple transcript variants encoding different isoforms have been found for this gene.

Product Overview

Entrez GenelD

9429

Aliases

MRX; MXR; ABCP; BCRP; BMDP; MXR1; ABC15; BCRP1; ABCG2; GOUT1; MXR-1; CDw338; UAQTL1; EST157481

Clone#

4H3C10

Host / Isotype

Mouse / IgG2b

Immunogen

Purified recombinant fragment of human CD338 (AA: extra mix) expressed in E. Coli.

Formulation

Purified antibody in PBS with 0.05% sodium azide

Storage

4℃; -20℃ for long term storage

Product Applications

WB (Western Blot)

1/500 - 1/2000

IHC_P(Immunohistochemistry)

1/200 - 1/1000

ICC (Immunocytochemistry)

1/200 - 1/1000

FCM (Flow Cytometry)

1/200 - 1/400

ELISA

1/10000

References

1,Nat Commun. 2020 May 8;11(1):2264.2,J Neuropathol Exp Neurol. 2020 Mar 1;79(3):266-276.

Product Image

Elisa

Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Western Blot

Figure 2:Western blot analysis using CD338 mAb against human CD338 (AA: extra mix) recombinant protein. (Expected MW is 24.2 kDa)

Western Blot

Figure 3:Western blot analysis using CD338 mAb against HEK293-6e (1) and CD338 (AA: extra mix)-hIgGFc transfected HEK293-6e (2) cell lysate.

Western Blot

Figure 4:Western blot analysis using CD338 mouse mAb against A549 (1), HePG2 (2), Hela (3), and NIH/3T3 (4) cell lysate.

Flow cytometric analysis

Figure 5:Flow cytometric analysis of Hela cells using CD338 mouse mAb (green) and negative control (red).

Flow cytometric analysis

Figure 6:Flow cytometric analysis of HePG2 cells using CD338 mouse mAb (green) and negative control (red).

Immunofluorescence analysis

Figure 7:Immunofluorescence analysis of Hela cells using CD338 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

For Research Use Only. Not for use in diagnostic procedures.