Mouse Monoclonal Antibody to CD317

Item Information

Catalog #

Size

Price

Description

Bone marrow stromal cells are involved in the growth and development of B-cells. The specific function of the protein encoded by the bone marrow stromal cell antigen 2 is undetermined; however, this protein may play a role in pre-B-cell growth and in rheumatoid arthritis.

Product Overview

Entrez GenelD

684

Aliases

BST2; TETHERIN

Clone#

7H2G12

Host / Isotype

Mouse / IgG2a

Immunogen

Purified recombinant fragment of human CD317 (AA: extra 49-161) expressed in E. Coli.

Formulation

Purified antibody in PBS with 0.05% sodium azide

Storage

4℃; -20℃ for long term storage

Product Applications

WB (Western Blot)

1/500 - 1/2000

IHC_P(Immunohistochemistry)

1/200 - 1/1000

ICC (Immunocytochemistry)

1/100 - 1/500

FCM (Flow Cytometry)

1/200 - 1/400

ELISA

1/10000

References

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Product Image

Elisa

Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Western Blot

Figure 2:Western blot analysis using CD317 mAb against human CD317 (AA: extra 49-161) recombinant protein. (Expected MW is 38.6 kDa)

Western Blot

Figure 3:Western blot analysis using CD317 mAb against HEK293-6e (1) and CD317 (AA: extra 49-161)-hIgGFc transfected HEK293-6e (2) cell lysate.

Immunofluorescence analysis

Figure 4:Immunofluorescence analysis of Hela cells using CD317 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Flow cytometric analysis