Mouse Monoclonal Antibody to CD300F
Item Information
Catalog #
Size
Price
Description
This gene encodes a member of the CD300 protein family. Members of this family are cell surface glycoproteins with a single IgV-like extracellular domain, and are involved in the regulation of immune response. The encoded protein is an inhibitory receptor. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]
Product Overview
Entrez GenelD
146722
Aliases
CLM1; NKIR; CLM-1; IREM1; LMIR3; CD300lf; IREM-1; IgSF13
Clone#
2F1E11
Host / Isotype
Mouse / IgG2b
Immunogen
Purified recombinant fragment of human CD300F (AA: Extra(20-156)) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4℃; -20℃ for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1,Brain Pathol. 2012 May;22(3):318-28. 2,J Immunol. 2007 Jan 15;178(2):808-16.
Product Image
Elisa
![Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/8c54757fc14a409f4ba87497d3254dbb5bc6f7fe6d54f8ecc6136f1471166334.jpg?v=1666705675)
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
![Figure 2:Western blot analysis using CD300F mAb against human CD300F (AA: Extra(20-156)) recombinant protein. (Expected MW is 41kDa)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/3d7e829873fec7682dbca9c91bba0ecb027fcf84a9ddb3509abea554f792d6f7.jpg?v=1666705675)
Figure 2:Western blot analysis using CD300F mAb against human CD300F (AA: Extra(20-156)) recombinant protein. (Expected MW is 41kDa)
Western Blot
![Figure 3:Western blot analysis using CD300F mAb against HEK293-6e (1) and CD300F (AA: Extra(20-156))-hIgGFc transfected HEK293-6e (2) cell lysate.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/ac4990f96cd2c0770fdb70e0640682093f48b0d029b90669d89b8a9c3e53bf69.jpg?v=1666705675)
Figure 3:Western blot analysis using CD300F mAb against HEK293-6e (1) and CD300F (AA: Extra(20-156))-hIgGFc transfected HEK293-6e (2) cell lysate.
Flow cytometric analysis
![Figure 4:Flow cytometric analysis of Jurkat cells using CD300F mouse mAb (green) and negative control (red).](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/ca0ce4dc342139fce9a3d6d4861e7993e90915f1e6241c72b00c4231b47fbfee.jpg?v=1666705675)
Figure 4:Flow cytometric analysis of Jurkat cells using CD300F mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.