Mouse Monoclonal Antibody to CD300F
Item Information
Catalog #
Size
Price
Description
This gene encodes a member of the CD300 protein family. Members of this family are cell surface glycoproteins with a single IgV-like extracellular domain, and are involved in the regulation of immune response. The encoded protein is an inhibitory receptor. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]
Product Overview
Entrez GenelD
146722
Aliases
CLM1; NKIR; CLM-1; IREM1; LMIR3; CD300lf; IREM-1; IgSF13
Clone#
2G1G7
Host / Isotype
Mouse / IgG2b
Immunogen
Purified recombinant fragment of human CD300F (AA: Extra(20-156)) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4℃; -20℃ for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1,Brain Pathol. 2012 May;22(3):318-28. 2,J Immunol. 2007 Jan 15;178(2):808-16.
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using CD300F mAb against human CD300F (AA: Extra(20-156)) recombinant protein. (Expected MW is 41kDa)
Western Blot
Figure 3:Western blot analysis using CD300F mAb against HEK293-6e (1) and CD300F (AA: Extra(20-156))-hIgGFc transfected HEK293-6e (2) cell lysate.
Flow cytometric analysis
Figure 4:Flow cytometric analysis of K562 cells using CD300F mouse mAb (green) and negative control (red).
Flow cytometric analysis
Figure 5:Flow cytometric analysis of Raji cells using CD300F mouse mAb (green) and negative control (red).
Flow cytometric analysis
Figure 6:Flow cytometric analysis of U937 cells using CD300F mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 7:Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using CD300F mouse mAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.