CD300C Primary Antibody
Item Information
Catalog #
Size
Price
Description
The CMRF35 antigen, which was identified by reactivity with a monoclonal antibody, is present on monocytes, neutrophils, and some T and B lymphocytes (Jackson et al., 1992 [PubMed 1349532]).
Product Overview
Entrez GenelD
10871
Aliases
LIR; CLM-6; CMRF35; IGSF16; CMRF-35; CMRF35A; CMRF-35A; CMRF35A1; CMRF35-A1
Clone#
5E1H8A5
Host / Isotype
Mouse / Mouse IgG2a
Immunogen
Purified recombinant fragment of human CD300C (AA: 21-183) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Product Applications
WB (Western Blot)
1/500 - 1/2000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Sci Rep. 2016 Apr 4;6:23942. 2.J Innate Immun. 2013;5(4):389-400.
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using CD300C mAb against human CD300C (AA: 21-183) recombinant protein. (Expected MW is 43.8 kDa)
Western Blot
Figure 3:Western blot analysis using CD300C mAb against HEK293 (1) and CD300C (AA: 21-183)-hIgGFc transfected HEK293 (2) cell lysate.
Flow cytometric
Figure 4:Flow cytometric analysis of THP-1 cells using CD300C mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.