Mouse Monoclonal Antibody to CD30

Item Information

Catalog #

Size

Price

Description

The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is expressed by activated, but not by resting, T and B cells. TRAF2 and TRAF5 can interact with this receptor, and mediate the signal transduction that leads to the activation of NF-kappaB. This receptor is a positive regulator of apoptosis, and also has been shown to limit the proliferative potential of autoreactive CD8 effector T cells and protect the body against autoimmunity. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.

Product Overview

Entrez GenelD

943

Aliases

TNFRSF8; Ki-1; D1S166E

Clone#

2A5A4

Host / Isotype

Mouse / IgG1

Immunogen

Purified recombinant fragment of human CD30 (AA: 416-595) expressed in E. Coli.

Formulation

Purified antibody in PBS with 0.05% sodium azide

Storage

4℃; -20℃ for long term storage

Product Applications

WB (Western Blot)

1/500 - 1/2000

ICC (Immunocytochemistry)

1/200 - 1/1000

FCM (Flow Cytometry)

1/200 - 1/400

ELISA

1/10000

References

1.Biomed Res Int. 2018 Jun 10;2018:3261436. 2.J Clin Invest. 2018 Jul 2;128(7):2996-3007.

Product Image

Elisa

Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Western Blot

Figure 2:Western blot analysis using CD30 mAb against human CD30 (AA: 416-595) recombinant protein. (Expected MW is 22.4 kDa)

Western Blot

Figure 3:Western blot analysis using CD30 mAb against HEK293-6e (1) and CD30 (AA: 416-595)-hIgGFc transfected HEK293-6e (2) cell lysate.

Western Blot

Figure 4:Western blot analysis using CD30 mouse mAb against Hela (1), MCF-7 (2), and HCT116 (3) cell lysate.

Immunofluorescence analysis

Figure 5:Immunofluorescence analysis of Hela cells using CD30 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Flow cytometric analysis