Mouse Monoclonal Antibody to CD30

Item Information
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Description

The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is expressed by activated, but not by resting, T and B cells. TRAF2 and TRAF5 can interact with this receptor, and mediate the signal transduction that leads to the activation of NF-kappaB. This receptor is a positive regulator of apoptosis, and also has been shown to limit the proliferative potential of autoreactive CD8 effector T cells and protect the body against autoimmunity. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.

Product Overview
Entrez GenelD
943
Aliases
TNFRSF8; Ki-1; D1S166E
Clone#
2A5A4
Host / Isotype
Mouse / IgG1
Immunogen
Purified recombinant fragment of human CD30 (AA: 416-595) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4℃; -20℃ for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
ICC (Immunocytochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Biomed Res Int. 2018 Jun 10;2018:3261436. 2.J Clin Invest. 2018 Jul 2;128(7):2996-3007.
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using CD30 mAb against human CD30 (AA: 416-595) recombinant protein. (Expected MW is 22.4 kDa)
Figure 2:Western blot analysis using CD30 mAb against human CD30 (AA: 416-595) recombinant protein. (Expected MW is 22.4 kDa)
Western Blot
Figure 3:Western blot analysis using CD30 mAb against HEK293-6e (1) and CD30 (AA: 416-595)-hIgGFc transfected HEK293-6e (2) cell lysate.
Figure 3:Western blot analysis using CD30 mAb against HEK293-6e (1) and CD30 (AA: 416-595)-hIgGFc transfected HEK293-6e (2) cell lysate.
Western Blot
Figure 4:Western blot analysis using CD30 mouse mAb against Hela (1), MCF-7 (2), and HCT116 (3) cell lysate.
Figure 4:Western blot analysis using CD30 mouse mAb against Hela (1), MCF-7 (2), and HCT116 (3) cell lysate.
Immunofluorescence analysis
Figure 5:Immunofluorescence analysis of Hela cells using CD30 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Figure 5:Immunofluorescence analysis of Hela cells using CD30 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric analysis
Figure 6:Flow cytometric analysis of Jurkat cells using CD30 mouse mAb (green) and negative control (red).
Figure 6:Flow cytometric analysis of Jurkat cells using CD30 mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.