CD30 Primary Antibody

Item Information
Catalog #
Size
Price
Description

The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is expressed by activated, but not by resting, T and B cells. TRAF2 and TRAF5 can interact with this receptor, and mediate the signal transduction that leads to the activation of NF-kappaB. This receptor is a positive regulator of apoptosis, and also has been shown to limit the proliferative potential of autoreactive CD8 effector T cells and protect the body against autoimmunity. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. 

Product Overview
Entrez GenelD
943
Aliases
CD30; Ki-1; D1S166E; TNFRSF8
Clone#
3B10
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human CD30 expressed in E. Coli.
Formulation
Ascitic fluid containing 0.03% sodium azide.
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1. Exp Clin Transplant. 2009 Dec;7(4):237-40.
2. Mol Vis. 2009 Oct 17;15:2068-73.
Product Image
Western Blot
Figure 1: Western blot analysis using CD30 mAb against human CD30 (AA: 536-590) recombinant protein. (Expected MW is 32 kDa)
Figure 1: Western blot analysis using CD30 mAb against human CD30 (AA: 536-590) recombinant protein. (Expected MW is 32 kDa)
Western Blot
Figure 2: Western blot analysis using CD30 mouse mAb against Hela cell lysate.
Figure 2: Western blot analysis using CD30 mouse mAb against Hela cell lysate.
Flow cytometric
Figure 3: Flow cytometric analysis of Hela cells using CD30 mouse mAb (green) and negative control (red).
Figure 3: Flow cytometric analysis of Hela cells using CD30 mouse mAb (green) and negative control (red).
Elisa
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
For Research Use Only. Not for use in diagnostic procedures.